GCLC ELISA Kit

Produktnummer ABIN5654641

Produktdetails GCLC ELISA Kit

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glutamate Cysteine Ligase, Catalytic (GCLC). No significant cross-reactivity or interference between Glutamate Cysteine Ligase, Catalytic (GCLC) and analogues was observed.

Sensitivität: 0.119 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Catalytic (GCLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Cysteine Ligase, Catalytic (GCLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: Glutamate Cysteine Ligase, Catalytic (GCLC Produkte)

Synonyme: GCLC ELISA Kit, gclc ELISA Kit, MGC146287 ELISA Kit, 2259 ELISA Kit, CG2259 ELISA Kit, DmGCLC ELISA Kit, DmGCS ELISA Kit, DmGCSh ELISA Kit, Dmel\\CG2259 ELISA Kit, GCL ELISA Kit, GCLc ELISA Kit, GCSh ELISA Kit, Gcs ELISA Kit, Gcsh ELISA Kit, DDBDRAFT_0186120 ELISA Kit, DDBDRAFT_0231403 ELISA Kit, DDB_0186120 ELISA Kit, DDB_0231403 ELISA Kit, GCS ELISA Kit, GLCL ELISA Kit, GLCLC ELISA Kit, D9Wsu168e ELISA Kit, GLCL-H ELISA Kit, Ggcs-hs ELISA Kit, Glclc ELISA Kit, cb1049 ELISA Kit, fe36e11 ELISA Kit, wu:fe36e11 ELISA Kit, glutamate-cysteine ligase catalytic subunit ELISA Kit, glutamate-cysteine ligase, catalytic subunit ELISA Kit, Glutamate-cysteine ligase catalytic subunit ELISA Kit, gamma glutamylcysteine synthetase ELISA Kit, glutamate-cysteine ligase, catalytic subunit L homeolog ELISA Kit, glutamate-cysteine ligase, catalytic subunit S homeolog ELISA Kit, gamma-glutamylcysteine synthetase ELISA Kit, GCLC ELISA Kit, gclc ELISA Kit, Gclc ELISA Kit, LbGCS ELISA Kit, gcsA ELISA Kit, gclc.L ELISA Kit, gclc.S ELISA Kit, GSH1 ELISA Kit

Hintergrund:

Gene Name: Glutamate Cysteine Ligase, Catalytic

Gene Aliases: GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS

Gen-ID: 14629

UniProt: P97494

Pathways: Cell RedoxHomeostasis