GBA ELISA Kit

Produktnummer ABIN5654636

Produktdetails GBA ELISA Kit

Target: GBA (Glucosidase, Beta, Acid (GBA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glucosidase Beta, Acid (GbA). No significant cross-reactivity or interference between Glucosidase Beta, Acid (GbA) and analogues was observed.

Sensitivität: 0.064 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Beta, Acid (GbA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Beta, Acid (GbA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Beta, Acid (GbA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucosidase Beta, Acid (GbA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucosidase Beta, Acid (GbA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucosidase Beta, Acid (GbA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GBA

Target: GBA (Glucosidase, Beta, Acid (GBA))

Andere Bezeichnung: Glucosidase Beta, Acid (GBA Produkte)

Synonyme: GBA1 ELISA Kit, GCB ELISA Kit, GLUC ELISA Kit, BETA-GLUCOSIDASE ELISA Kit, T12J13.8 ELISA Kit, T12J13_8 ELISA Kit, beta glucosidase 25 ELISA Kit, PSPTO3318 ELISA Kit, PSPTO4290 ELISA Kit, GC ELISA Kit, GCase ELISA Kit, betaGC ELISA Kit, glucosylceramidase beta ELISA Kit, beta glucosidase 25 ELISA Kit, beta-glucosidase ELISA Kit, Beta-glucosidase ELISA Kit, glucosidase, beta, acid ELISA Kit, glucosylceramidase ELISA Kit, bglX-2 ELISA Kit, GBA ELISA Kit, BGLU25 ELISA Kit, PSPTO_3318 ELISA Kit, bglX ELISA Kit, bglA4 ELISA Kit, Gba ELISA Kit, LOC100399524 ELISA Kit, bglA.2 ELISA Kit, bglX-2 ELISA Kit

Hintergrund:

Gene Name: Glucosidase Beta, Acid

Gene Aliases: GBA1, GCB, GLUC, Imiglucerase, Alglucerase, Beta-glucocerebrosidase, Glucosylceramidase, β-Glucosidase, D-Glucosyl-N-Acylsphingosine Glucohydrolase

Gen-ID: 2629

UniProt: P04062

Pathways: Cellular Glucan Metabolic Process