GDI1 ELISA Kit

Produktnummer ABIN5654540

Produktdetails GDI1 ELISA Kit

Target: GDI1 (GDP Dissociation Inhibitor 1 (GDI1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of GDP Dissociation Inhibitor 1 (GDI1). No significant cross-reactivity or interference between GDP Dissociation Inhibitor 1 (GDI1) and analogues was observed.

Sensitivität: 0.052 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GDP Dissociation Inhibitor 1 (GDI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GDP Dissociation Inhibitor 1 (GDI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDP Dissociation Inhibitor 1 (GDI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDP Dissociation Inhibitor 1 (GDI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GDI1

Target: GDI1 (GDP Dissociation Inhibitor 1 (GDI1))

Andere Bezeichnung: GDP Dissociation Inhibitor 1 (GDI1 Produkte)

Synonyme: 1A ELISA Kit, GDIL ELISA Kit, MRX41 ELISA Kit, MRX48 ELISA Kit, OPHN2 ELISA Kit, RABGD1A ELISA Kit, RABGDIA ELISA Kit, XAP-4 ELISA Kit, GDIA ELISA Kit, GDIalpha ELISA Kit, GDI-1 ELISA Kit, zgc:110600 ELISA Kit, QccE-14779 ELISA Kit, Rab GDI alpha ELISA Kit, GDP dissociation inhibitor 1 ELISA Kit, GDP dissociation inhibitor 1 S homeolog ELISA Kit, guanosine diphosphate (GDP) dissociation inhibitor 1 ELISA Kit, GDI1 ELISA Kit, gdi1.S ELISA Kit, Gdi1 ELISA Kit, gdi1 ELISA Kit

Hintergrund:

Gene Name: GDP Dissociation Inhibitor 1

Gene Aliases: GDIL, MRX41, MRX48, OPHN2, RABGD1A, RABGDIA, XAP-4, Guanine Nucleotide Dissociation Inhibitor, Rab GDP Dissociation Inhibitor Alpha 1, Oligophrenin-2

Gen-ID: 2664

UniProt: P31150