GGH ELISA Kit

Produktnummer ABIN5654503

Produktdetails GGH ELISA Kit

Target: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Gamma-Glutamyl Hydrolase (gGH). No significant cross-reactivity or interference between Gamma-Glutamyl Hydrolase (gGH) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyl Hydrolase (gGH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Glutamyl Hydrolase (gGH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyl Hydrolase (gGH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-Glutamyl Hydrolase (gGH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Glutamyl Hydrolase (gGH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Glutamyl Hydrolase (gGH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GGH

Target: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Andere Bezeichnung: Gamma-Glutamyl Hydrolase (GGH Produkte)

Synonyme: CK2A2 ELISA Kit, CSNK2A1 ELISA Kit, GH ELISA Kit, gamma-GH ELISA Kit, zgc:76958 ELISA Kit, wu:fa56a05 ELISA Kit, wu:fj90a01 ELISA Kit, MGC84044 ELISA Kit, GGH ELISA Kit, MGC130662 ELISA Kit, ATGGH2 ELISA Kit, gamma-glutamyl hydrolase 2 ELISA Kit, casein kinase 2 alpha 2 ELISA Kit, gamma-glutamyl hydrolase ELISA Kit, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) ELISA Kit, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) L homeolog ELISA Kit, gamma-glutamyl hydrolase 2 ELISA Kit, Gamma-glutamyl hydrolase ELISA Kit, CSNK2A2 ELISA Kit, GGH ELISA Kit, Ggh ELISA Kit, ggh ELISA Kit, ggh.L ELISA Kit, ggh.2.L ELISA Kit, GGH2 ELISA Kit, GH ELISA Kit

Hintergrund:

Gene Name: Gamma-Glutamyl Hydrolase

Gene Aliases: GH, conjugase, folylpolygammaglutamyl hydrolase, Gamma-Glu-X carboxypeptidase

Gen-ID: 8836

UniProt: Q92820

Pathways: SARS-CoV-2 Protein Interaktom