Galanin ELISA Kit
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- Target Alle Galanin (GAL) ELISA Kits anzeigen
- Galanin (GAL)
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Competition ELISA
- Detektionsbereich
- 12.35 pg/mL - 1000 pg/mL
- Untere Nachweisgrenze
- 12.35 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Galanin (GAL). No significant cross-reactivity or interference between Galanin (GAL) and analogues was observed.
- Sensitivität
- 4.97 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 h
- Plattentyp
- Pre-coated
- Protokoll
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Galanin (GAL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Galanin (GAL) and unlabeled Galanin (GAL) (Standards or samples) with the pre-coated antibody specific to Galanin (GAL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Galanin (GAL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Galanin (GAL) in the sample.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle Galanin (GAL) ELISA Kits anzeigen
- Galanin (GAL)
- Andere Bezeichnung
- Galanin (GAL Produkte)
- Synonyme
- Galn ELISA Kit, galanin and GMAP prepropeptide ELISA Kit, galanin ELISA Kit, GAL ELISA Kit, Gal ELISA Kit
- Hintergrund
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Gene Name: Galanin
Gene Aliases: GALN, GLNN, GMAP, Galanin Prepropeptide, Galanin-Message-Associated Peptide
- Gen-ID
- 29141
- UniProt
- P10683
- Pathways
- Regulation of Hormone Metabolic Process, Feeding Behaviour
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