Galanin CLIA Kit

Produktnummer ABIN5654471

Human Galanin ELISA Kit, Chemiluminescent Assay für die Quantifizierung von Human Galanin.

Kurzübersicht für Galanin CLIA Kit (ABIN5654471)

Target: Galanin (GAL)

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 11.72 pg/mL - 3000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Galanin CLIA Kit

Untere Nachweisgrenze: 11.72 pg/mL

Verwendungszweck: Human Galanin (GAL) CLIA Kit for detecting GAL in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Galanin (GAL). No significant cross-reactivity or interference between Galanin (GAL) and analogues was observed.

Sensitivität: 4.23 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Galanin (GAL). A competitive inhibition reaction is launched between biotin labeled Galanin (GAL) and unlabeled Galanin (GAL) (Standards or samples) with the pre-coated antibody specific to Galanin (GAL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Galanin (GAL) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Galanin (GAL) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Galanin (GAL)

Andere Bezeichnung: Galanin

Hintergrund: Gene Aliases: GALN, GLNN, GMAP, Galanin Prepropeptide, Galanin-Message-Associated Peptide

UniProt: P22466

Pathways: Regulation of Hormone Metabolic Process, Feeding Behaviour