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FUT3 ELISA Kit

FUT3 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5654439
  • Target Alle FUT3 ELISA Kits anzeigen
    FUT3 (Fucosyltransferase 3 (Galactoside 3(4)-L-Fucosyltransferase, Lewis Blood Group) (FUT3))
    Reaktivität
    • 3
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Fucosyltransferase 3 (FUT3). No significant cross-reactivity or interference between Fucosyltransferase 3 (FUT3) and analogues was observed.
    Sensitivität
    0.062 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosyltransferase 3 (FUT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosyltransferase 3 (FUT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosyltransferase 3 (FUT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosyltransferase 3 (FUT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosyltransferase 3 (FUT3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosyltransferase 3 (FUT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle FUT3 ELISA Kits anzeigen
    FUT3 (Fucosyltransferase 3 (Galactoside 3(4)-L-Fucosyltransferase, Lewis Blood Group) (FUT3))
    Andere Bezeichnung
    Fucosyltransferase 3 (FUT3 Produkte)
    Synonyme
    CD174 ELISA Kit, FT3B ELISA Kit, FucT-III ELISA Kit, LE ELISA Kit, Les ELISA Kit, fut356 ELISA Kit, FUT3 ELISA Kit, ATFUT3 ELISA Kit, F1M20.10 ELISA Kit, F1M20_10 ELISA Kit, fucosyltransferase 3 ELISA Kit, fucosyltransferase 3 (Lewis blood group) ELISA Kit, fucosyltransferase 3 (Lewis blood group) L homeolog ELISA Kit, fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) ELISA Kit, fucosyltransferase 3 ELISA Kit, galactoside 3(4)-L-fucosyltransferase ELISA Kit, FUT3 ELISA Kit, fut3.L ELISA Kit, fut3 ELISA Kit, LOC697452 ELISA Kit, LOC697582 ELISA Kit
    Hintergrund

    Gene Name: Fucosyltransferase 3

    Gene Aliases: CD174, FUT3, FT3B, LE, Les, FucT-III, Lewis FT, Galactoside 3(4)-L-Fucosyltransferase, Lewis Blood Group, Blood group Lewis alpha-4-fucosyltransferase

    Gen-ID
    2525
    UniProt
    P21217
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