FOXA2 ELISA Kit

Produktnummer ABIN5654407

Produktdetails FOXA2 ELISA Kit

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein A2 (FOXA2). No significant cross-reactivity or interference between Forkhead Box Protein A2 (FOXA2) and analogues was observed.

Sensitivität: 0.126 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein A2 (FOXA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein A2 (FOXA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein A2 (FOXA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein A2 (FOXA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein A2 (FOXA2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein A2 (FOXA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Andere Bezeichnung: Forkhead Box Protein A2 (FOXA2 Produkte)

Synonyme: hnf3b ELISA Kit, tcf3b ELISA Kit, hnf3beta ELISA Kit, hnf3-beta ELISA Kit, FOXA2 ELISA Kit, HNF3B ELISA Kit, TCF3B ELISA Kit, HNF-3B ELISA Kit, ax ELISA Kit, axl ELISA Kit, fkd1 ELISA Kit, ik:tdsubc_2g8 ELISA Kit, tdsubc_2g8 ELISA Kit, wu:fe05g04 ELISA Kit, xx:tdsubc_2g8 ELISA Kit, FoxA2-A ELISA Kit, FoxA2a ELISA Kit, xFD-3 ELISA Kit, xbeta-1 ELISA Kit, HNF-3b ELISA Kit, HNF3-beta ELISA Kit, HNF3beta ELISA Kit, Hnf-3b ELISA Kit, Hnf3b ELISA Kit, Tcf-3b ELISA Kit, Tcf3b ELISA Kit, HNF-3BETA ELISA Kit, forkhead box A2 ELISA Kit, forkhead box A2 L homeolog ELISA Kit, foxa2 ELISA Kit, FOXA2 ELISA Kit, foxa2.L ELISA Kit, Foxa2 ELISA Kit

Hintergrund:

Gene Name: Forkhead Box Protein A2

Gene Aliases: FOX-A2, HNF3B, TCF3B, Hepatocyte Nuclear Factor 3,Beta, Transcription factor 3B

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process