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FKBP1B ELISA Kit

FKBP1B Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5654358
  • Target Alle FKBP1B ELISA Kits anzeigen
    FKBP1B (FK506 Binding Protein 1B, 12.6 KDa (FKBP1B))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 1B (FKBP1B). No significant cross-reactivity or interference between FK506 Binding Protein 1B (FKBP1B) and analogues was observed.
    Sensitivität
    0.053 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1B (FKBP1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1B (FKBP1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1B (FKBP1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 1B (FKBP1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle FKBP1B ELISA Kits anzeigen
    FKBP1B (FK506 Binding Protein 1B, 12.6 KDa (FKBP1B))
    Andere Bezeichnung
    FK506 Binding Protein 1B (FKBP1B Produkte)
    Synonyme
    FKBP12.6 ELISA Kit, MGC68829 ELISA Kit, FKBP1B ELISA Kit, DKFZp459K1429 ELISA Kit, 12.6kDa ELISA Kit, AW494148 ELISA Kit, calstabin2 ELISA Kit, FKBP1L ELISA Kit, OTK4 ELISA Kit, PKBP1L ELISA Kit, PPIase ELISA Kit, FK506 binding protein 1B ELISA Kit, FK506 binding protein 1B L homeolog ELISA Kit, FK506 binding protein 1b ELISA Kit, FKBP1B ELISA Kit, fkbp1b.L ELISA Kit, fkbp1b ELISA Kit, Fkbp1b ELISA Kit
    Hintergrund

    Gene Name: FK506 Binding Protein 1B

    Gene Aliases: FKBP12.6, FKBP1L, FKBP9, OTK4, PKBP1L, Rotamase, 12.6 kDa FK506-binding protein, Immunophilin FKBP12.6, Peptidyl-prolyl cis-trans isomerase FKBP1B

    Gen-ID
    2281
    UniProt
    P68106
    Pathways
    Hormone Transport, Negative Regulation of Hormone Secretion, Negative Regulation of Transporter Activity
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