Filamin A ELISA Kit

Produktnummer ABIN5654350

Produktdetails Filamin A ELISA Kit

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.88 ng/mL - 120 ng/mL

Untere Nachweisgrenze: 1.88 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Filamin A Alpha (FLNa). No significant cross-reactivity or interference between Filamin A Alpha (FLNa) and analogues was observed.

Sensitivität: 0.65 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha (FLNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin A Alpha (FLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha (FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha (FLNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin A Alpha (FLNa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin A Alpha (FLNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Filamin A

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Andere Bezeichnung: Filamin A Alpha (FLNA Produkte)

Synonyme: ABP-280 ELISA Kit, ABPX ELISA Kit, CSBS ELISA Kit, CVD1 ELISA Kit, FLN ELISA Kit, FLN-A ELISA Kit, FLN1 ELISA Kit, FMD ELISA Kit, MNS ELISA Kit, NHBP ELISA Kit, OPD ELISA Kit, OPD1 ELISA Kit, OPD2 ELISA Kit, XLVD ELISA Kit, XMVD ELISA Kit, fb98b06 ELISA Kit, wu:fb98b06 ELISA Kit, wu:fd09g03 ELISA Kit, wu:fd49a06 ELISA Kit, Dilp2 ELISA Kit, F730004A14Rik ELISA Kit, Fln1 ELISA Kit, filamin-1 ELISA Kit, RGD1560614 ELISA Kit, MGC147094 ELISA Kit, CG3937 ELISA Kit, Cher ELISA Kit, Dmel\\CG3937 ELISA Kit, Fil ELISA Kit, joy ELISA Kit, sko ELISA Kit, filamin A ELISA Kit, filamin A, alpha (actin binding protein 280) ELISA Kit, filamin, alpha ELISA Kit, filamin-A ELISA Kit, cheerio ELISA Kit, FLNA ELISA Kit, flna ELISA Kit, Flna ELISA Kit, LOC587317 ELISA Kit, cher ELISA Kit, LOC100380643 ELISA Kit

Hintergrund:

Gene Name: Filamin A Alpha

Gene Aliases: ABP280, FLN-A, ABPX, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, Actin Binding Protein 280, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin

Gen-ID: 2316

UniProt: P21333

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location