FGF18 ELISA Kit

Produktnummer ABIN5654226

Produktdetails FGF18 ELISA Kit

Target: FGF18 (Fibroblast Growth Factor 18 (FGF18))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 18 (FGF18). No significant cross-reactivity or interference between Fibroblast Growth Factor 18 (FGF18) and analogues was observed.

Sensitivität: 5.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 18 (FGF18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 18 (FGF18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 18 (FGF18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 18 (FGF18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FGF18

Target: FGF18 (Fibroblast Growth Factor 18 (FGF18))

Andere Bezeichnung: Fibroblast Growth Factor 18 (FGF18 Produkte)

Synonyme: FGF-18 ELISA Kit, ZFGF5 ELISA Kit, D130055P09Rik ELISA Kit, fgf18 ELISA Kit, FGF18 ELISA Kit, fgf18l ELISA Kit, fibroblast growth factor 18 ELISA Kit, fibroblast growth factor 18a ELISA Kit, fibroblast growth factor 18b ELISA Kit, FGF18 ELISA Kit, Fgf18 ELISA Kit, fgf18a ELISA Kit, Tsp_09844 ELISA Kit, fgf18b ELISA Kit

Hintergrund:

Gene Name: Fibroblast Growth Factor 18

Gene Aliases: ZFGF5

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung