Fatty Acid Synthase ELISA Kit

Produktnummer ABIN5654094

Produktdetails Fatty Acid Synthase ELISA Kit

Target: Fatty Acid Synthase (FASN)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN). No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) and analogues was observed.

Sensitivität: 0.057 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Synthase (FASN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Synthase (FASN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Fatty Acid Synthase

Target: Fatty Acid Synthase (FASN)

Andere Bezeichnung: Fatty Acid Synthase (FASN Produkte)

Synonyme: FAS ELISA Kit, OA-519 ELISA Kit, SDR27X1 ELISA Kit, FASN ELISA Kit, fj34h12 ELISA Kit, wu:fj34h12 ELISA Kit, Cgl0836 ELISA Kit, A630082H08Rik ELISA Kit, BcDNA:GH07626 ELISA Kit, BcDNA:gh07626 ELISA Kit, DM_7295848 ELISA Kit, Dmel\\CG3523 ELISA Kit, Fatty acid synthase ELISA Kit, anon-EST:Posey235 ELISA Kit, anon-EST:Posey43 ELISA Kit, anon-WO0138581.1 ELISA Kit, dFAS ELISA Kit, fas ELISA Kit, fatty acid synthase ELISA Kit, fatty acid synthase Fas ELISA Kit, type I polyketide synthase ELISA Kit, Fatty acid synthase Fas ELISA Kit, Fatty Acid SyNthase ELISA Kit, fatty-acid synthase ELISA Kit, fatty-acid synthase II ELISA Kit, fatty-acid synthase fas ELISA Kit, Fatty acid synthase 1 ELISA Kit, FASN ELISA Kit, fas ELISA Kit, Fasn ELISA Kit, BAD_RS01360 ELISA Kit, fasn ELISA Kit, fasn-1 ELISA Kit, NCgl0802 ELISA Kit, fasB ELISA Kit, MT2600 ELISA Kit, TBMG_01448 ELISA Kit, FASN1 ELISA Kit

Hintergrund:

Gene Name: Fatty Acid Synthase

Gene Aliases: FAS, OA519, SDR27X1, Short Chain Dehydrogenase/Reductase Family 27X,Member 1, S-acetyltransferase, 3-oxoacyl synthase, Oleoyl hydrolase, Enoyl-acyl-carrier-protein reductase

Gen-ID: 14104

UniProt: P19096

Pathways: AMPK Signaling