Estrogen Receptor alpha ELISA Kit

Produktnummer ABIN5653989

Produktdetails Estrogen Receptor alpha ELISA Kit

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Alpha (ERa). No significant cross-reactivity or interference between Estrogen Receptor Alpha (ERa) and analogues was observed.

Sensitivität: 0.126 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Alpha (ERa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Alpha (ERa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Alpha (ERa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Alpha (ERa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: Estrogen Receptor Alpha (ESR1 Produkte)

Synonyme: LOC398734 ELISA Kit, AA420328 ELISA Kit, AU041214 ELISA Kit, ER-alpha ELISA Kit, ER[a] ELISA Kit, ERa ELISA Kit, ERalpha ELISA Kit, ESR ELISA Kit, Estr ELISA Kit, Estra ELISA Kit, Nr3a1 ELISA Kit, NR3A1 ELISA Kit, eralpha ELISA Kit, zfER[a] ELISA Kit, ER ELISA Kit, ESRA ELISA Kit, ESTRR ELISA Kit, Era ELISA Kit, akap12 ELISA Kit, cb27 ELISA Kit, esr1 ELISA Kit, fb72g12 ELISA Kit, id:ibd1202 ELISA Kit, sb:cb27 ELISA Kit, si:ch73-192g21.1 ELISA Kit, wu:fb72g12 ELISA Kit, wu:fc21c03 ELISA Kit, Esr ELISA Kit, RNESTROR ELISA Kit, ERALPHA ELISA Kit, er ELISA Kit, esr ELISA Kit, nr3a1 ELISA Kit, XER ELISA Kit, era ELISA Kit, esra ELISA Kit, ERbeta ELISA Kit, xesr-1 ELISA Kit, xlERalpha1 ELISA Kit, xlERalpha2 ELISA Kit, ESR1 ELISA Kit, estrogen receptor 1 L homeolog ELISA Kit, estrogen receptor 1 (alpha) ELISA Kit, estrogen receptor 1 ELISA Kit, A kinase (PRKA) anchor protein 12b ELISA Kit, esr1.L ELISA Kit, Esr1 ELISA Kit, ESR1 ELISA Kit, esr1 ELISA Kit, akap12b ELISA Kit

Hintergrund:

Gene Name: Estrogen Receptor Alpha

Gene Aliases: ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1

Gen-ID: 24890

UniProt: P06211

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly