Endothelin 1 CLIA Kit

Produktnummer ABIN5653858

Das Human Endothelin 1 ELISA Kit (ABIN5653858) ist ein Chemiluminescent ELISA Kit zur Detektion von Human Endothelin 1.

Kurzübersicht für Endothelin 1 CLIA Kit (ABIN5653858)

Target: Endothelin 1 (EDN1)

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 3.9 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Endothelin 1 CLIA Kit

Untere Nachweisgrenze: 3.9 pg/mL

Verwendungszweck: Human Endothelin 1 (EDN1) CLIA Kit for detecting EDN1 in serum, plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.

Sensitivität: 1.4 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Endothelin 1 (EDN1). A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) and unlabeled Endothelin 1 (EDN1) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Endothelin 1 (EDN1) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Endothelin 1 (EDN1)

Andere Bezeichnung: Endothelin 1

Hintergrund: Gene Aliases: ET1, PPET1, Preproendothelin-1, Big endothelin-1

Gen-ID: 1906

UniProt: P05305

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size