ETFB ELISA Kit

Produktnummer ABIN5653817

Produktdetails ETFB ELISA Kit

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Beta Polypeptide (ETFb). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Beta Polypeptide (ETFb) and analogues was observed.

Sensitivität: 0.112 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Beta Polypeptide (ETFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Beta Polypeptide (ETFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Electron Transfer Flavoprotein Beta Polypeptide (ETFb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Electron Transfer Flavoprotein Beta Polypeptide (ETFb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ETFB

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Andere Bezeichnung: Electron Transfer Flavoprotein Beta Polypeptide (ETFB Produkte)

Synonyme: MWF20.14 ELISA Kit, MWF20_14 ELISA Kit, electron transfer flavoprotein beta ELISA Kit, 0610009I16Rik ELISA Kit, 2810441H06Rik ELISA Kit, MADD ELISA Kit, electron transfer flavoprotein beta ELISA Kit, electron transfer flavoprotein subunit beta ELISA Kit, electron transferring flavoprotein, beta polypeptide ELISA Kit, electron transfer flavoprotein beta subunit L homeolog ELISA Kit, electron transfer flavoprotein beta subunit ELISA Kit, ETFBETA ELISA Kit, FixA ELISA Kit, Etfb ELISA Kit, etfb.L ELISA Kit, ETFB ELISA Kit

Hintergrund:

Gene Name: Electron Transfer Flavoprotein Beta Polypeptide

Gene Aliases: ETF-B, MADD, FP585

Gen-ID: 2109

UniProt: P38117