Dystroglycan ELISA Kit

Produktnummer ABIN5653764

Produktdetails Dystroglycan ELISA Kit

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Dystrophin Associated Glycoprotein 1 (DAG1). No significant cross-reactivity or interference between Dystrophin Associated Glycoprotein 1 (DAG1) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrophin Associated Glycoprotein 1 (DAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrophin Associated Glycoprotein 1 (DAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Andere Bezeichnung: Dystrophin Associated Glycoprotein 1 (DAG1 Produkte)

Synonyme: LOC398500 ELISA Kit, dag1 ELISA Kit, MGC53537 ELISA Kit, 156DAG ELISA Kit, A3a ELISA Kit, AGRNR ELISA Kit, DAG ELISA Kit, MDDGC7 ELISA Kit, MDDGC9 ELISA Kit, DAG1 ELISA Kit, RAB7 ELISA Kit, dg ELISA Kit, a3a ELISA Kit, dag ELISA Kit, agrnr ELISA Kit, 156dag ELISA Kit, D9Wsu13e ELISA Kit, DG ELISA Kit, Dp427 ELISA Kit, Dp71 ELISA Kit, CG18250 ELISA Kit, CT41273 ELISA Kit, DmDG ELISA Kit, Dmel\\CG18250 ELISA Kit, atu ELISA Kit, dgn ELISA Kit, dys ELISA Kit, GB14967 ELISA Kit, APOJ ELISA Kit, CLI ELISA Kit, RATTRPM2B ELISA Kit, SGP-2 ELISA Kit, SGP2 ELISA Kit, SP-40 ELISA Kit, SP40 ELISA Kit, TRPM-2 ELISA Kit, TRPM2B ELISA Kit, Trpm2 ELISA Kit, Trpmb ELISA Kit, Ala-1 ELISA Kit, H9/25 ELISA Kit, Ly-27 ELISA Kit, Ly-6 ELISA Kit, Ly27 ELISA Kit, wu:fb83d06 ELISA Kit, wu:fi25f06 ELISA Kit, wu:fi37b08 ELISA Kit, zgc:109786 ELISA Kit, DystroGlycaN ELISA Kit, dystroglycan 1 L homeolog ELISA Kit, dystroglycan 1 ELISA Kit, dystroglycan 1 S homeolog ELISA Kit, Dystroglycan ELISA Kit, dystroglycan ELISA Kit, clusterin ELISA Kit, lymphocyte antigen 6 complex ELISA Kit, dgn-1 ELISA Kit, dag1.L ELISA Kit, dgn-2 ELISA Kit, dgn-3 ELISA Kit, DAG1 ELISA Kit, dag1.S ELISA Kit, Dag1 ELISA Kit, dag1 ELISA Kit, Dg ELISA Kit, LOC408826 ELISA Kit, Clu ELISA Kit, Ly6 ELISA Kit

Hintergrund:

Gene Name: Dystrophin Associated Glycoprotein 1

Gene Aliases: Dystroglycan 1, 156DAG, A3a, AGRNR, DAG, Alpha-Dystroglycan, Beta-Dystroglycan

Gen-ID: 13138

UniProt: Q62165

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus