Dopamine d2 Receptor ELISA Kit
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- Target Alle Dopamine d2 Receptor (DRD2) ELISA Kits anzeigen
- Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Dopamine Receptor D2 (DRD2). No significant cross-reactivity or interference between Dopamine Receptor D2 (DRD2) and analogues was observed.
- Sensitivität
- 0.123 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D2 (DRD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D2 (DRD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D2 (DRD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Receptor D2 (DRD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Receptor D2 (DRD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Receptor D2 (DRD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle Dopamine d2 Receptor (DRD2) ELISA Kits anzeigen
- Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))
- Andere Bezeichnung
- Dopamine Receptor D2 (DRD2 Produkte)
- Synonyme
- D2DR ELISA Kit, D2R ELISA Kit, drd2b ELISA Kit, d2r2 ELISA Kit, Drd-2 ELISA Kit, d2dr ELISA Kit, d2r ELISA Kit, drd2 ELISA Kit, drd2-B ELISA Kit, DRD2 ELISA Kit, D2 ELISA Kit, CG18741 ELISA Kit, CG7569 ELISA Kit, DAMB ELISA Kit, DDR2 ELISA Kit, DOPR2 ELISA Kit, DOPR99B ELISA Kit, Dar2 ELISA Kit, Dmel\\CG18741 ELISA Kit, DopD1 ELISA Kit, DopR99B ELISA Kit, DopRII ELISA Kit, dopamine receptor D2 ELISA Kit, dopamine receptor D2 like ELISA Kit, dopamine receptor D2 L homeolog ELISA Kit, dopamine receptor D2 S homeolog ELISA Kit, Dopamine 1-like receptor 2 ELISA Kit, DRD2 ELISA Kit, drd2l ELISA Kit, drd2 ELISA Kit, Drd2 ELISA Kit, drd2.L ELISA Kit, drd2.S ELISA Kit, Dop1R2 ELISA Kit
- Hintergrund
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Gene Name: Dopamine Receptor D2
Gene Aliases: DR-D2, D2DR, D2R
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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