Dystroglycan ELISA Kit

Produktnummer ABIN5653647

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Diverse Spezies Dystroglycan.

Kurzübersicht für Dystroglycan ELISA Kit (ABIN5653647)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 391 pg/mL - 100000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Dystroglycan ELISA Kit

Untere Nachweisgrenze: 391 pg/mL

Verwendungszweck: Diacylglycerol (DAG) ELISA Kit for detecting DAG in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Diacylglycerol (DAG). No significant cross-reactivity or interference between Diacylglycerol (DAG) and analogues was observed.

Sensitivität: 153 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Diacylglycerol (DAG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Diacylglycerol (DAG) and unlabeled Diacylglycerol (DAG) (Standards or samples) with the pre-coated antibody specific to Diacylglycerol (DAG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Diacylglycerol (DAG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Diacylglycerol (DAG) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol (DAG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol (DAG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Andere Bezeichnung: Diacylglycerol

Hintergrund: Gene Aliases: DG, Diglyceride

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus