POR ELISA Kit

Produktnummer ABIN5653524

Produktdetails POR ELISA Kit

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 Reductase (CPR). No significant cross-reactivity or interference between Cytochrome P450 Reductase (CPR) and analogues was observed.

Sensitivität: 0.107 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Reductase (CPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Reductase (CPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Reductase (CPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 Reductase (CPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu POR

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Andere Bezeichnung: Cytochrome P450 Reductase (POR Produkte)

Synonyme: CPR ELISA Kit, CYPOR ELISA Kit, P450R ELISA Kit, POR ELISA Kit, CCR ELISA Kit, CG11567 ELISA Kit, DMR ELISA Kit, DmCPR ELISA Kit, Dmel\\CG11567 ELISA Kit, NCPR ELISA Kit, P450 ELISA Kit, cpr ELISA Kit, zgc:110032 ELISA Kit, npr ELISA Kit, xpor ELISA Kit, 4933424M13Rik ELISA Kit, PORCINO ELISA Kit, T5J17.90 ELISA Kit, T5J17_90 ELISA Kit, TFC C ELISA Kit, TUBULIN-FOLDING COFACTOR C ELISA Kit, cytochrome p450 oxidoreductase ELISA Kit, P450 (cytochrome) oxidoreductase ELISA Kit, ADP-ribosylation factor interacting protein 2 ELISA Kit, Cytochrome P450 reductase ELISA Kit, P450 (cytochrome) oxidoreductase a ELISA Kit, P450 (cytochrome) oxidoreductase L homeolog ELISA Kit, NADPH--cytochrome P450 reductase ELISA Kit, C-CAP/cofactor C-like domain-containing protein ELISA Kit, POR ELISA Kit, Arfip2 ELISA Kit, Por ELISA Kit, Cpr ELISA Kit, pora ELISA Kit, por.L ELISA Kit, LOC100406157 ELISA Kit, por ELISA Kit, LOC100619047 ELISA Kit

Hintergrund:

Gene Name: Cytochrome P450 Reductase

Gene Aliases: POR, CYPOR, P450R, P450(Cytochrome)Oxidoreductase, NADPH--cytochrome P450 reductase

Gen-ID: 29441

UniProt: P00388

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interaktom