COX IV ELISA Kit

Produktnummer ABIN5653486

Produktdetails COX IV ELISA Kit

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Andere Bezeichnung: Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1 Produkte)

Synonyme: COX4 ELISA Kit, COX4-1 ELISA Kit, COXIV ELISA Kit, mg:bb02d03 ELISA Kit, zgc:110058 ELISA Kit, GB20012 ELISA Kit, AL024441 ELISA Kit, Cox4 ELISA Kit, Cox4a ELISA Kit, cox4 ELISA Kit, cox4i1 ELISA Kit, coxiv ELISA Kit, BcDNA:AT07685 ELISA Kit, CG10396 ELISA Kit, COX IV ELISA Kit, CX41 ELISA Kit, CX42 ELISA Kit, Dmel\CG10396 ELISA Kit, IV ELISA Kit, cytochrome c oxidase subunit 4I1 ELISA Kit, cytochrome c oxidase subunit IV isoform 1 ELISA Kit, cytochrome c oxidase subunit 4 isoform 1, mitochondrial ELISA Kit, cytochrome c oxidase polypeptide IV ELISA Kit, cytochrome c oxidase subunit IV ELISA Kit, cytochrome c oxidase subunit 4i1 ELISA Kit, cytochrome c oxidase subunit IV isoform 1 S homeolog ELISA Kit, Cytochrome c oxidase subunit 4-like ELISA Kit, COX4I1 ELISA Kit, cox4i1 ELISA Kit, LOC412396 ELISA Kit, Cox4I1 ELISA Kit, LOC780848 ELISA Kit, BMEII0322 ELISA Kit, RSal33209_1582 ELISA Kit, SJAG_00976 ELISA Kit, HMPREF0733_11926 ELISA Kit, Cox4i1 ELISA Kit, cox4i1.S ELISA Kit, COX4L ELISA Kit

Hintergrund:

Gene Name: Cytochrome C Oxidase Subunit IV Isoform 1

Gene Aliases: COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Pathways: Proton Transport