COX2 ELISA Kit

Produktnummer ABIN5653483

Produktdetails COX2 ELISA Kit

Target: COX2 (Cytochrome C Oxidase Subunit II (COX2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit II (COX2). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit II (COX2) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit II (COX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit II (COX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit II (COX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit II (COX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit II (COX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit II (COX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu COX2

Target: COX2 (Cytochrome C Oxidase Subunit II (COX2))

Andere Bezeichnung: Cytochrome C Oxidase Subunit II (COX2 Produkte)

Synonyme: COX-2 ELISA Kit, Cox2 ELISA Kit, COII ELISA Kit, MTCO2 ELISA Kit, cox ELISA Kit, SC6G10.29c ELISA Kit, cCOII ELISA Kit, prostaglandin-endoperoxide synthase 2 ELISA Kit, cytochrome c oxidase subunit II ELISA Kit, COXII ELISA Kit, cytochrome c oxidase subunit ii ELISA Kit, cytochrome C oxidase subunit II ELISA Kit, cytochrome c oxidase subunit 2 ELISA Kit, cytochrome oxidase subunit 2 ELISA Kit, Ptgs2 ELISA Kit, COX2 ELISA Kit, Smed_0518 ELISA Kit, Mrub_0709 ELISA Kit, Ocepr_1683 ELISA Kit, SCO2156 ELISA Kit, coxB ELISA Kit, Dtpsy_2860 ELISA Kit, Ndas_3150 ELISA Kit, Mesil_0628 ELISA Kit, Bresu_0801 ELISA Kit, cox2 ELISA Kit

Hintergrund:

Gene Name: Cytochrome C Oxidase Subunit II

Gene Aliases: MTCO2, MT-CO2, COII, COXII, Mitochondrially Encoded Cytochrome C Oxidase II, Cytochrome c oxidase polypeptide II

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation