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CDK16 ELISA Kit

CDK16 Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5653418
  • Target Alle CDK16 ELISA Kits anzeigen
    CDK16 (Cyclin-Dependent Kinase 16 (CDK16))
    Reaktivität
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.25 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.25 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 16 (CDK16). No significant cross-reactivity or interference between Cyclin Dependent Kinase 16 (CDK16) and analogues was observed.
    Sensitivität
    12.6 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 16 (CDK16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 16 (CDK16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 16 (CDK16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 16 (CDK16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase 16 (CDK16) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase 16 (CDK16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CDK16 ELISA Kits anzeigen
    CDK16 (Cyclin-Dependent Kinase 16 (CDK16))
    Andere Bezeichnung
    Cyclin Dependent Kinase 16 (CDK16 Produkte)
    Synonyme
    PCTAIRE ELISA Kit, PCTAIRE1 ELISA Kit, PCTGAIRE ELISA Kit, PCTK1 ELISA Kit, Crk5 ELISA Kit, Pctaire1 ELISA Kit, Pctk1 ELISA Kit, pctk1 ELISA Kit, pctaire ELISA Kit, pctaire1 ELISA Kit, pctgaire ELISA Kit, cyclin dependent kinase 16 ELISA Kit, cyclin-dependent kinase 16 ELISA Kit, cyclin-dependent kinase 16 S homeolog ELISA Kit, CDK16 ELISA Kit, Cdk16 ELISA Kit, cdk16 ELISA Kit, cdk16.S ELISA Kit
    Hintergrund

    Gene Name: Cyclin Dependent Kinase 16

    Gene Aliases: PCTK1, PCTAIRE, PCTAIRE1, PCTGAIRE, Serine/Threonine-Protein Kinase PCTAIRE-1, PCTAIRE-motif protein kinase 1, Cell division protein kinase 16

    Gen-ID
    5127
    UniProt
    Q00536
    Pathways
    Hormone Transport
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