Vitamin B12 ELISA Kit

Produktnummer ABIN5653404

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Diverse Spezies Vitamin B12.

Kurzübersicht für Vitamin B12 ELISA Kit (ABIN5653404)

Target: Vitamin B12

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 123.5 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails Vitamin B12 ELISA Kit

Untere Nachweisgrenze: 123.5 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cyanocobalamin (CNCbl). No significant cross-reactivity or interference between Cyanocobalamin (CNCbl) and analogues was observed.

Sensitivität: 49.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cyanocobalamin (CNCbl) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyanocobalamin (CNCbl) and unlabeled Cyanocobalamin (CNCbl) (Standards or samples) with the pre-coated antibody specific to Cyanocobalamin (CNCbl). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyanocobalamin (CNCbl) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyanocobalamin (CNCbl) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Vitamin B12

Target: Vitamin B12

Andere Bezeichnung: Cyanocobalamin

Substanzklasse: Chemical

Hintergrund:

Gene Name: Cyanocobalamin

Gene Aliases: VB12, Vitamin B12, Cobalamin, α-5,6-Dimethylbenzimidazolyl Cobamidcyanide