CBP ELISA Kit

Produktnummer ABIN5653371

Produktdetails CBP ELISA Kit

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of CREB Binding Protein (CREBBP). No significant cross-reactivity or interference between CREB Binding Protein (CREBBP) and analogues was observed.

Sensitivität: 0.132 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CREB Binding Protein (CREBBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CREB Binding Protein (CREBBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CREB Binding Protein (CREBBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CREB Binding Protein (CREBBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CREB Binding Protein (CREBBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CREB Binding Protein (CREBBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CBP

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Andere Bezeichnung: CREB Binding Protein (CREBBP Produkte)

Synonyme: CBP ELISA Kit, KAT3A ELISA Kit, RSTS ELISA Kit, AW558298 ELISA Kit, CBP/p300 ELISA Kit, p300/CBP ELISA Kit, RTS ELISA Kit, cbp ELISA Kit, crebbp ELISA Kit, kat3a ELISA Kit, rsts ELISA Kit, hmm291030 ELISA Kit, Nejire ELISA Kit, CREB binding protein ELISA Kit, CREB binding protein L homeolog ELISA Kit, histone acetyltransferase p300 ELISA Kit, CREBBP ELISA Kit, Crebbp ELISA Kit, crebbp.L ELISA Kit, LOC100123220 ELISA Kit

Hintergrund:

Gene Name: CREB Binding Protein

Gene Aliases: CBP, RSTS, RTS, KAT3A, Rubinstein-Taybi Syndrome

Gen-ID: 1387

UniProt: Q92793

Pathways: T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha