CRH ELISA Kit (Corticotropin Releasing Hormone)

Details for Product CRH ELISA Kit No. ABIN5653338, Anbieter: Anmelden zum Anzeigen
  • CRF
  • Gm1347
  • crf
  • crh
  • zgc:101864
  • CRH
  • corticoliberin
  • crhb
  • crh.1-a
  • crha
  • corticotropin releasing hormone
  • corticotropin releasing hormone b
  • corticotropin releasing hormone S homeolog
  • corticotropin releasing hormone L homeolog
  • Crh
  • CRH
  • crhb
  • crh
  • crh.S
  • crh.L
Kits mit alternativen Reaktivitäten:
Competition ELISA
12.35 pg/mL - 1000 pg/mL
Untere Nachweisgrenze
12.35 pg/mL
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Hersteller Produkt- Nr.
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Proben Plasma, Serum
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone (CRH). No significant cross-reactivity or interference between Corticotropin Releasing Hormone (CRH) and analogues was observed.
Sensitivität 4.91 pg/mL
Plasmids, Primers & others Plasmide, Primers & weitere CRH products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung Corticotropin Releasing Hormone (CRH ELISA Kit Abstract)

Gene Name: Corticotropin Releasing Hormone

Gene Aliases: CRF, Corticotropin Releasing Factor, Corticoliberin

Gen-ID 1392
UniProt P06850
Pathways Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer 2 h
Plattentyp Pre-coated
Protokoll This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticotropin Releasing Hormone (CRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corticotropin Releasing Hormone (CRH) and unlabeled Corticotropin Releasing Hormone (CRH) (Standards or samples) with the pre-coated antibody specific to Corticotropin Releasing Hormone (CRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticotropin Releasing Hormone (CRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticotropin Releasing Hormone (CRH) in the sample.
Testpräzision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Hormone (CRH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Hormone (CRH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Haltbarkeit 4-8 months
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