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GJB2 ELISA Kit

GJB2 Reaktivität: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5653300
  • Target Alle GJB2 ELISA Kits anzeigen
    GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))
    Reaktivität
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
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    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Connexin 26 (CX26). No significant cross-reactivity or interference between Connexin 26 (CX26) and analogues was observed.
    Sensitivität
    0.66 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 26 (CX26). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connexin 26 (CX26). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 26 (CX26), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connexin 26 (CX26) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connexin 26 (CX26) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connexin 26 (CX26) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle GJB2 ELISA Kits anzeigen
    GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))
    Andere Bezeichnung
    Connexin 26 (GJB2 Produkte)
    Synonyme
    ppk ELISA Kit, cx26 ELISA Kit, dfna3 ELISA Kit, dfnb1 ELISA Kit, nsrd1 ELISA Kit, dfna3a ELISA Kit, dfnb1a ELISA Kit, MGC53062 ELISA Kit, connexin-26 ELISA Kit, GJB2 ELISA Kit, AI325222 ELISA Kit, Cnx26 ELISA Kit, Cx26 ELISA Kit, Gjb-2 ELISA Kit, CXN-26 ELISA Kit, CX26 ELISA Kit, DFNA3 ELISA Kit, DFNA3A ELISA Kit, DFNB1 ELISA Kit, DFNB1A ELISA Kit, HID ELISA Kit, KID ELISA Kit, NSRD1 ELISA Kit, PPK ELISA Kit, gap junction protein beta 2 L homeolog ELISA Kit, gap junction protein beta 2 ELISA Kit, gap junction protein, beta 2 ELISA Kit, gjb2.L ELISA Kit, GJB2 ELISA Kit, Gjb2 ELISA Kit
    Hintergrund

    Gene Name: Connexin 26

    Gene Aliases: KID, DFNA3, DFNB1, HID, NSRD1, PPK, GJB2, Gap Junction Protein Beta 2

    Pathways
    Sensory Perception of Sound, Cell-Cell Junction Organization
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