CTGF ELISA Kit

Produktnummer ABIN5653299

Produktdetails CTGF ELISA Kit

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Sensitivität: 12.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connective Tissue Growth Factor (CTGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connective Tissue Growth Factor (CTGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Andere Bezeichnung: Connective Tissue Growth Factor (CTGF Produkte)

Synonyme: Ccn2 ELISA Kit, Fisp12 ELISA Kit, Hcs24 ELISA Kit, fisp-12 ELISA Kit, CCN2 ELISA Kit, HCS24 ELISA Kit, IGFBP8 ELISA Kit, NOV2 ELISA Kit, CTGRP ELISA Kit, ctgf ELISA Kit, hm:zeh1559 ELISA Kit, si:dkey-266k12.4 ELISA Kit, wu:fl25c03 ELISA Kit, zeh1559 ELISA Kit, zgc:136644 ELISA Kit, XCTGF ELISA Kit, ccn2 ELISA Kit, nov2 ELISA Kit, hcs24 ELISA Kit, igfbp8 ELISA Kit, CTGF ELISA Kit, si:ch211-173b8.2 ELISA Kit, connective tissue growth factor ELISA Kit, connective tissue growth factor a ELISA Kit, connective tissue growth factor L homeolog ELISA Kit, connective tissue growth factor b ELISA Kit, Ctgf ELISA Kit, CTGF ELISA Kit, ctgfa ELISA Kit, ctgf.L ELISA Kit, ctgf ELISA Kit, ctgfb ELISA Kit

Hintergrund:

Gene Name: Connective Tissue Growth Factor

Gene Aliases: CCN2, HCS24, IGFBP8, NOV2, Insulin-Like Growth Factor-Binding Protein 8, Hypertrophic Chondrocyte-Specific Protein 24, CCN Family Member 2

Gen-ID: 64032

UniProt: Q9R1E9

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding