Cofilin ELISA Kit

Produktnummer ABIN5653076

Produktdetails Cofilin ELISA Kit

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cofilin 1, Non Muscle (CFL1). No significant cross-reactivity or interference between Cofilin 1, Non Muscle (CFL1) and analogues was observed.

Sensitivität: 0.057 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cofilin 1, Non Muscle (CFL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cofilin 1, Non Muscle (CFL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cofilin 1, Non Muscle (CFL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cofilin 1, Non Muscle (CFL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cofilin 1, Non Muscle (CFL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cofilin 1, Non Muscle (CFL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin 1, Non Muscle (CFL1 Produkte)

Synonyme: cfl1 ELISA Kit, xac1 ELISA Kit, xac2 ELISA Kit, cfl ELISA Kit, cb86 ELISA Kit, sb:cb86 ELISA Kit, zgc:56501 ELISA Kit, wu:fb18a04 ELISA Kit, wu:fk77b03 ELISA Kit, 12/8 ELISA Kit, ADF ELISA Kit, ADF/cofilin ELISA Kit, Adf ELISA Kit, CG4254 ELISA Kit, Cadf ELISA Kit, D-cof ELISA Kit, D61 ELISA Kit, Dmel\\CG4254 ELISA Kit, T8 ELISA Kit, Tsr ELISA Kit, cofilin ELISA Kit, cofilin/ADF ELISA Kit, cofilin/actin depolymerizing factor homolog ELISA Kit, l(2)03101 ELISA Kit, l(2)k03101 ELISA Kit, l(2)k05633 ELISA Kit, l(2)k13409 ELISA Kit, ntf ELISA Kit, CFL ELISA Kit, AA959946 ELISA Kit, Cof ELISA Kit, CFL1 ELISA Kit, cofilin 1 (non-muscle) L homeolog ELISA Kit, cofilin 1 (non-muscle) ELISA Kit, cofilin 1 (non-muscle), like ELISA Kit, cofilin 1 ELISA Kit, twinstar ELISA Kit, cofilin ELISA Kit, cofilin 1, non-muscle ELISA Kit, Contrafreeloading ELISA Kit, cfl1.L ELISA Kit, cfl1 ELISA Kit, cfl1l ELISA Kit, CFL1 ELISA Kit, tsr ELISA Kit, COF1 ELISA Kit, TSTA_042790 ELISA Kit, Cfl1 ELISA Kit, CFL ELISA Kit

Hintergrund:

Gene Name: Cofilin 1, Non Muscle

Gene Aliases: CFL, p18, N-Cofilin, 18 kDa phosphoprotein

Gen-ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events