Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Coagulation Factor X ELISA Kit

F10 Reaktivität: Human Colorimetric Sandwich ELISA 0.188 ng/mL - 12 ng/mL Plasma
Produktnummer ABIN5653057
  • Target Alle Coagulation Factor X (F10) ELISA Kits anzeigen
    Coagulation Factor X (F10)
    Reaktivität
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.188 ng/mL - 12 ng/mL
    Untere Nachweisgrenze
    0.188 ng/mL
    Applikation
    ELISA
    Proben
    Plasma
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Coagulation Factor X (F10). No significant cross-reactivity or interference between Coagulation Factor X (F10) and analogues was observed.
    Sensitivität
    0.09 ng/mL
    Featured
    Zu unserem meistverkauften F10 ELISA Kit
    Top Product
    Discover our top product F10 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor X (F10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor X (F10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor X (F10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor X (F10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor X (F10) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor X (F10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Coagulation Factor X (F10) ELISA Kits anzeigen
    Coagulation Factor X (F10)
    Andere Bezeichnung
    Coagulation Factor X (F10 Produkte)
    Synonyme
    FX ELISA Kit, FXA ELISA Kit, Cf10 ELISA Kit, fX ELISA Kit, fi12c10 ELISA Kit, wu:fi12c10 ELISA Kit, F10 ELISA Kit, f10 ELISA Kit, coagulation factor X ELISA Kit, Coagulation factor X ELISA Kit, coagulation factor 10 L homeolog ELISA Kit, F10 ELISA Kit, f10 ELISA Kit, CpipJ_CPIJ012712 ELISA Kit, CpipJ_CPIJ014863 ELISA Kit, CpipJ_CPIJ016937 ELISA Kit, CpipJ_CPIJ017791 ELISA Kit, fa10 ELISA Kit, f10.L ELISA Kit
    Hintergrund

    Gene Name: Coagulation Factor X

    Gene Aliases: FX, FXA, Stuart Prower Factor, Thrombokinase

    Gen-ID
    2159
    UniProt
    P00742
Sie sind hier:
Kundenservice