CLOCK ELISA Kit

Produktnummer ABIN5652968

Produktdetails CLOCK ELISA Kit

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Circadian Locomoter Output Cycles Protein Kaput (CLOCK). No significant cross-reactivity or interference between Circadian Locomoter Output Cycles Protein Kaput (CLOCK) and analogues was observed.

Sensitivität: 0.117 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Circadian Locomoter Output Cycles Protein Kaput (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Circadian Locomoter Output Cycles Protein Kaput (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Andere Bezeichnung: Circadian Locomoter Output Cycles Protein Kaput (CLOCK Produkte)

Synonyme: CG7391 ELISA Kit, CLK ELISA Kit, CLOCK ELISA Kit, Dmel\\CG7391 ELISA Kit, Jerk ELISA Kit, Jrk ELISA Kit, PAS1 ELISA Kit, bHLHe10 ELISA Kit, clk ELISA Kit, clock ELISA Kit, dCLK ELISA Kit, dCLK/JRK ELISA Kit, dCLOCK ELISA Kit, dClck ELISA Kit, dClk ELISA Kit, dClock ELISA Kit, dclk ELISA Kit, jrk ELISA Kit, Xclk ELISA Kit, XClock ELISA Kit, zCLOCK1 ELISA Kit, zfCLOCK1 ELISA Kit, 5330400M04Rik ELISA Kit, KAT13D ELISA Kit, bHLHe8 ELISA Kit, mKIAA0334 ELISA Kit, Clock ELISA Kit, clock circadian regulator ELISA Kit, clock homolog (mouse) ELISA Kit, clock circadian regulator a ELISA Kit, circadian locomotor output cycles kaput ELISA Kit, clock circadian regulator L homeolog ELISA Kit, Clk ELISA Kit, CLOCK ELISA Kit, clock ELISA Kit, clocka ELISA Kit, Clock ELISA Kit, clock.L ELISA Kit

Hintergrund:

Gene Name: Circadian Locomoter Output Cycles Protein Kaput

Gene Aliases: KAT13D, bHLHe8, Class E basic helix-loop-helix protein 8

Gen-ID: 9575

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism