Chromogranin A ELISA Kit

Produktnummer ABIN5652945

Produktdetails Chromogranin A ELISA Kit

Target: Chromogranin A (CHGA)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125.00 pg/mL - 8000 pg/mL

Untere Nachweisgrenze: 125.00 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Chromogranin A (CHGA). No significant cross-reactivity or interference between Chromogranin A (CHGA) and analogues was observed.

Sensitivität: 48 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromogranin A (CHGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromogranin A (CHGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chromogranin A (CHGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromogranin A (CHGA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromogranin A (CHGA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Chromogranin A

Target: Chromogranin A (CHGA)

Andere Bezeichnung: Chromogranin A (CHGA Produkte)

Synonyme: CGA ELISA Kit, CG-ALPHA ELISA Kit, FSHA ELISA Kit, GPHA1 ELISA Kit, GPHa ELISA Kit, HCG ELISA Kit, LHA ELISA Kit, TSHA ELISA Kit, ChrA ELISA Kit, fj05f09 ELISA Kit, si:dkey-177p2.2 ELISA Kit, wu:fj05f09 ELISA Kit, zgc:101749 ELISA Kit, zgc:56075 ELISA Kit, CHGA ELISA Kit, cgA ELISA Kit, chga ELISA Kit, chromogranin A ELISA Kit, glycoprotein hormones, alpha polypeptide ELISA Kit, uncharacterized CHGA ELISA Kit, chromogranin A S homeolog ELISA Kit, CHGA ELISA Kit, CGA ELISA Kit, Chga ELISA Kit, chga ELISA Kit, chga.S ELISA Kit

Hintergrund:

Gene Name: Chromogranin A

Gene Aliases: CGA, SP-I, Parathyroid Secretory Protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I

Gen-ID: 12652

UniProt: P26339

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling