Cathelicidin ELISA Kit

Produktnummer ABIN5652685

Produktdetails Cathelicidin ELISA Kit

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cathelicidin Antimicrobial Peptide (CAMP). No significant cross-reactivity or interference between Cathelicidin Antimicrobial Peptide (CAMP) and analogues was observed.

Sensitivität: 0.27 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide (CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathelicidin Antimicrobial Peptide (CAMP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathelicidin Antimicrobial Peptide (CAMP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Cathelicidin

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Andere Bezeichnung: Cathelicidin Antimicrobial Peptide (CAMP Produkte)

Synonyme: CAP-18 ELISA Kit, CAP18 ELISA Kit, CRAMP ELISA Kit, FALL-39 ELISA Kit, FALL39 ELISA Kit, LL37 ELISA Kit, CATH-2 ELISA Kit, Cnlp ELISA Kit, Cramp ELISA Kit, MCLP ELISA Kit, CATH-B1 ELISA Kit, CATHB1 ELISA Kit, CATHL2 ELISA Kit, CATHL7 ELISA Kit, CAP11 ELISA Kit, cathelicidin antimicrobial peptide ELISA Kit, CAMP ELISA Kit, LOC100136187 ELISA Kit, LOC100136439 ELISA Kit, Camp ELISA Kit

Hintergrund:

Gene Name: Cathelicidin Antimicrobial Peptide

Gene Aliases: CAP18, FALL39, HSD26, LL37, 18 kDa Cationic Antimicrobial Protein, Antibacterial protein FALL-39, FALL-39 peptide antibiotic

Gen-ID: 820

UniProt: P49913

Pathways: Cellular Response to Molecule of Bacterial Origin