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CASP14 ELISA Kit

CASP14 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Produktnummer ABIN5652649
  • Target Alle CASP14 ELISA Kits anzeigen
    CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))
    Reaktivität
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Caspase 14 (CASP14). No significant cross-reactivity or interference between Caspase 14 (CASP14) and analogues was observed.
    Sensitivität
    0.117 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 14 (CASP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 14 (CASP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 14 (CASP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 14 (CASP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CASP14 ELISA Kits anzeigen
    CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))
    Andere Bezeichnung
    Caspase 14 (CASP14 Produkte)
    Synonyme
    CASP14 ELISA Kit, MICE ELISA Kit, mini-ICE ELISA Kit, caspase 14, apoptosis-related cysteine peptidase ELISA Kit, caspase 14 ELISA Kit, caspase-14-like ELISA Kit, caspase-14 ELISA Kit, CASP14 ELISA Kit, Casp14 ELISA Kit, LOC485601 ELISA Kit, LOC780455 ELISA Kit
    Hintergrund

    Gene Name: Caspase 14

    Gene Aliases: Caspase 14 Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 14

    Gen-ID
    299587
    UniProt
    D3ZZ65
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