CPT1A ELISA Kit
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- Target Alle CPT1A ELISA Kits anzeigen
- CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.78 ng/mL - 50 ng/mL
- Untere Nachweisgrenze
- 0.78 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Carnitine Palmitoyltransferase 1A, Liver (CPT1A). No significant cross-reactivity or interference between Carnitine Palmitoyltransferase 1A, Liver (CPT1A) and analogues was observed.
- Sensitivität
- 0.28 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Palmitoyltransferase 1A, Liver (CPT1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Palmitoyltransferase 1A, Liver (CPT1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Palmitoyltransferase 1A, Liver (CPT1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Palmitoyltransferase 1A, Liver (CPT1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Palmitoyltransferase 1A, Liver (CPT1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Palmitoyltransferase 1A, Liver (CPT1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle CPT1A ELISA Kits anzeigen
- CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))
- Andere Bezeichnung
- Carnitine Palmitoyltransferase 1A, Liver (CPT1A Produkte)
- Synonyme
- MGC53498 ELISA Kit, CPT1 ELISA Kit, CPT1-L ELISA Kit, L-CPT1 ELISA Kit, L-CPTI ELISA Kit, si:dkey-56p7.8 ELISA Kit, C730027G07 ELISA Kit, CPTI ELISA Kit, Cpt1 ELISA Kit, CPT-Ia ELISA Kit, carnitine palmitoyltransferase 1A (liver) L homeolog ELISA Kit, carnitine palmitoyltransferase 1A ELISA Kit, carnitine palmitoyltransferase 1A (liver) ELISA Kit, carnitine palmitoyltransferase 1Aa (liver) ELISA Kit, carnitine palmitoyltransferase 1a, liver ELISA Kit, cpt1a.L ELISA Kit, CPT1A ELISA Kit, cpt1a ELISA Kit, cpt1aa ELISA Kit, Cpt1a ELISA Kit
- Hintergrund
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Gene Name: Carnitine Palmitoyltransferase 1A, Liver
Gene Aliases: CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform
- Gen-ID
- 1374
- UniProt
- P50416
- Pathways
- AMPK Signaling, Regulation of Lipid Metabolism by PPARalpha, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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