CA2 ELISA Kit

Produktnummer ABIN5652551

Produktdetails CA2 ELISA Kit

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Huhn

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase II (CA2). No significant cross-reactivity or interference between Carbonic Anhydrase II (CA2) and analogues was observed.

Sensitivität: 0.59 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase II (CA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase II (CA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase II (CA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase II (CA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase II (CA2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase II (CA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: Carbonic Anhydrase II (CA2 Produkte)

Synonyme: CG6906 ELISA Kit, CT21171 ELISA Kit, Dmel\\CG6906 ELISA Kit, CA-2 ELISA Kit, caII ELISA Kit, car2 ELISA Kit, ca ii ELISA Kit, ca-ii ELISA Kit, BETA CA2 ELISA Kit, BETA CARBONIC ANHYDRASE 2 ELISA Kit, CA18 ELISA Kit, CARBONIC ANHYDRASE 18 ELISA Kit, CARBONIC ANHYDRASE 2 ELISA Kit, T9L3.40 ELISA Kit, T9L3_40 ELISA Kit, carbonic anhydrase 2 ELISA Kit, LOC100135827 ELISA Kit, AI131712 ELISA Kit, CAII ELISA Kit, Ca2 ELISA Kit, Car-2 ELISA Kit, Ltw-5 ELISA Kit, Lvtw-5 ELISA Kit, CA-II ELISA Kit, CAC ELISA Kit, Car2 ELISA Kit, CAR3 ELISA Kit, Carbonic anhydrase 2 ELISA Kit, carbonic anhydrase 2 L homeolog ELISA Kit, carbonic anhydrase 2 ELISA Kit, carbonic anhydrase 1 ELISA Kit, carbonic anhydrase II ELISA Kit, carbonic anhydrase, alpha type, periplasmic ELISA Kit, CAH2 ELISA Kit, ca2.L ELISA Kit, ca2 ELISA Kit, CA2 ELISA Kit, HCAG_04637 ELISA Kit, LOC100135827 ELISA Kit, LOC100635510 ELISA Kit, Car2 ELISA Kit, Ca2 ELISA Kit, LOC100154873 ELISA Kit, LOC101113341 ELISA Kit

Hintergrund:

Gene Name: Carbonic Anhydrase II

Gene Aliases: CA-II, CAII, Car2, CAC, Carbonate dehydratase II, Carbonic anhydrase C

Gen-ID: 396257

UniProt: P07630