CGRP CLIA Kit

Produktnummer ABIN5652477

Produktdetails CGRP CLIA Kit

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 7.81 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 7.81 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.

Sensitivität: 2.77 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP). A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Calcitonin Gene Related Peptide (CGRP) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Andere Bezeichnung: Calcitonin Gene Related Peptide (CALCA Produkte)

Synonyme: CALC1 CLIA Kit, CGRP CLIA Kit, CGRP-I CLIA Kit, CGRP1 CLIA Kit, CT CLIA Kit, KC CLIA Kit, CALC CLIA Kit, CALCA CLIA Kit, CALC3 CLIA Kit, ci-ct CLIA Kit, calca CLIA Kit, CA CLIA Kit, CGRP-1 CLIA Kit, Calc CLIA Kit, Calc1 CLIA Kit, Cgrp CLIA Kit, Ct CLIA Kit, Ctn CLIA Kit, calcitonin CLIA Kit, CAL6 CLIA Kit, Cal1 CLIA Kit, RATCAL6 CLIA Kit, CGRPI CLIA Kit, calc CLIA Kit, cgrp CLIA Kit, zgc:92886 CLIA Kit, calcitonin related polypeptide alpha CLIA Kit, calcitonin related polypeptide beta CLIA Kit, calcitonin-related polypeptide alpha CLIA Kit, calcitonin CLIA Kit, calcitonin/calcitonin-related polypeptide, alpha CLIA Kit, calcitonin-related polypeptide beta CLIA Kit, calcitonin gene-related peptide 2 CLIA Kit, CALCA CLIA Kit, CALCB CLIA Kit, ci-ct CLIA Kit, calca CLIA Kit, Calca CLIA Kit, LOC100008771 CLIA Kit

Hintergrund:

Gene Name: Calcitonin Gene Related Peptide

Gene Aliases: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta

UniProt: Q99MP3

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour