CTBP2 ELISA Kit

Produktnummer ABIN5652417

Produktdetails CTBP2 ELISA Kit

Target: CTBP2 (C-terminal Binding Protein 2 (CTBP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of C-Terminal Binding Protein 2 (CTBP2). No significant cross-reactivity or interference between C-Terminal Binding Protein 2 (CTBP2) and analogues was observed.

Sensitivität: 0.125 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Terminal Binding Protein 2 (CTBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Terminal Binding Protein 2 (CTBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Terminal Binding Protein 2 (CTBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Terminal Binding Protein 2 (CTBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Terminal Binding Protein 2 (CTBP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Terminal Binding Protein 2 (CTBP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CTBP2

Target: CTBP2 (C-terminal Binding Protein 2 (CTBP2))

Andere Bezeichnung: C-Terminal Binding Protein 2 (CTBP2 Produkte)

Synonyme: ctbp ELISA Kit, ctbp-b ELISA Kit, xctbp ELISA Kit, AA407280 ELISA Kit, D7Ertd45e ELISA Kit, Gtrgeo6 ELISA Kit, Ribeye ELISA Kit, fe05b01 ELISA Kit, wu:fb96g03 ELISA Kit, wu:fe05b01 ELISA Kit, wu:fk19e02 ELISA Kit, C-terminal binding protein 2 ELISA Kit, C-terminal-binding protein 2 ELISA Kit, C-terminal binding protein 2a ELISA Kit, C-terminal binding protein 2 S homeolog ELISA Kit, ctbp2 ELISA Kit, LOC100398309 ELISA Kit, CTBP2 ELISA Kit, Ctbp2 ELISA Kit, ctbp2a ELISA Kit, ctbp2.S ELISA Kit

Hintergrund:

Gene Name: C-Terminal Binding Protein 2

Gene Aliases: Ribeye

Gen-ID: 1488

UniProt: P56545