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BNP ELISA Kit

BNP Reaktivität: Maus Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5652323
  • Target Alle BNP ELISA Kits anzeigen
    BNP (Brain Natriuretic Peptide (BNP))
    Reaktivität
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    12.35 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    12.35 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Brain Natriuretic Peptide (BNP). No significant cross-reactivity or interference between Brain Natriuretic Peptide (BNP) and analogues was observed.
    Sensitivität
    4.81 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Brain Natriuretic Peptide (BNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Brain Natriuretic Peptide (BNP) and unlabeled Brain Natriuretic Peptide (BNP) (Standards or samples) with the pre-coated antibody specific to Brain Natriuretic Peptide (BNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Brain Natriuretic Peptide (BNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Brain Natriuretic Peptide (BNP) in the sample.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle BNP ELISA Kits anzeigen
    BNP (Brain Natriuretic Peptide (BNP))
    Andere Bezeichnung
    Brain Natriuretic Peptide (BNP Produkte)
    Synonyme
    bnp ELISA Kit, BNP1 ELISA Kit, NPPB ELISA Kit, BNP ELISA Kit, Bnf ELISA Kit, AA408272 ELISA Kit, B-type natriuretic peptide ELISA Kit, natriuretic peptide B ELISA Kit, natriuretic peptide type B ELISA Kit, nppb ELISA Kit, NPPB ELISA Kit, Nppb ELISA Kit
    Hintergrund

    Gene Name: Brain Natriuretic Peptide

    Gene Aliases: GC-B, NPPB, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-brain natriuretic peptide, Brain natriuretic peptide 32

    Gen-ID
    18158
    UniProt
    P40753
    Pathways
    Hormone Activity
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