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BMP5 ELISA Kit

BMP5 Reaktivität: Human Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5652282
  • Target Alle BMP5 ELISA Kits anzeigen
    BMP5 (Bone Morphogenetic Protein 5 (BMP5))
    Reaktivität
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.62 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    15.62 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 5 (BMP5). No significant cross-reactivity or interference between Bone Morphogenetic Protein 5 (BMP5) and analogues was observed.
    Sensitivität
    5.3 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 5 (BMP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 5 (BMP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 5 (BMP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 5 (BMP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 5 (BMP5) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 5 (BMP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle BMP5 ELISA Kits anzeigen
    BMP5 (Bone Morphogenetic Protein 5 (BMP5))
    Andere Bezeichnung
    Bone Morphogenetic Protein 5 (BMP5 Produkte)
    Synonyme
    bmp5l ELISA Kit, hm:zehl0669 ELISA Kit, zehl0669 ELISA Kit, zgc:64230 ELISA Kit, BMP5 ELISA Kit, AU023399 ELISA Kit, se ELISA Kit, bone morphogenetic protein 5 ELISA Kit, bmp5 ELISA Kit, BMP5 ELISA Kit, LOC100539495 ELISA Kit, Bmp5 ELISA Kit
    Hintergrund

    Gene Name: Bone Morphogenetic Protein 5

    Gen-ID
    653
    UniProt
    P22003
    Pathways
    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process
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