Big Dynorphin ELISA Kit

Produktnummer ABIN5652222

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Big Dynorphin.

Kurzübersicht für Big Dynorphin ELISA Kit (ABIN5652222)

Target: Big Dynorphin

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 6.17 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Big Dynorphin ELISA Kit

Untere Nachweisgrenze: 6.17 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Big Dynorphin (Dyn). No significant cross-reactivity or interference between Big Dynorphin (Dyn) and analogues was observed.

Sensitivität: 2.56 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Big Dynorphin (Dyn) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Big Dynorphin (Dyn) and unlabeled Big Dynorphin (Dyn) (Standards or samples) with the pre-coated antibody specific to Big Dynorphin (Dyn). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Big Dynorphin (Dyn) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Big Dynorphin (Dyn) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Big Dynorphin (Dyn) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Big Dynorphin (Dyn) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Big Dynorphin

Target: Big Dynorphin

Hintergrund:

Gene Name: Big Dynorphin

Gene Aliases: B-DYN, Biocytin 13 Dynorphin A 1-13 Amide