BHBA ELISA Kit

Produktnummer ABIN5652201

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Diverse Spezies BHBA.

Kurzübersicht für BHBA ELISA Kit (ABIN5652201)

Target: BHBA (beta-hydroxybutyric Acid (BHBA))

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 1.23 μg/mL - 100 μg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails BHBA ELISA Kit

Untere Nachweisgrenze: 1.23 μg/mL

Verwendungszweck: Beta-Hydroxybutyric Acid (bHB) ELISA Kit for detecting bHB in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Beta-Hydroxybutyric Acid (bHB). No significant cross-reactivity or interference between Beta-Hydroxybutyric Acid (bHB) and analogues was observed.

Sensitivität: 0.44 μg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Hydroxybutyric Acid (bHB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Hydroxybutyric Acid (bHB) and unlabeled Beta-Hydroxybutyric Acid (bHB) (Standards or samples) with the pre-coated antibody specific to Beta-Hydroxybutyric Acid (bHB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Hydroxybutyric Acid (bHB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Hydroxybutyric Acid (bHB) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu BHBA

Target: BHBA (beta-hydroxybutyric Acid (BHBA))

Andere Bezeichnung: Beta-Hydroxybutyric Acid

Hintergrund: Gene Aliases: OHB, BHBA, Beta-Hydroxybutyrate, 3-Hydroxybutyric Acid, β-Hydroxybutyric acid