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ARSF ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ARSF.
Produktnummer ABIN5652036

Kurzübersicht für ARSF ELISA Kit (ABIN5652036)

Target

Alle ARSF Produkte
ARSF (Arylsulfatase F (ARSF))

Reaktivität

Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

0.312 ng/mL - 20 ng/mL

Applikation

ELISA

Proben

Plasma, Serum, Tissue Homogenate
  • Untere Nachweisgrenze

    0.312 ng/mL

    Analytische Methode

    Quantitative

    Spezifität

    This assay has high sensitivity and excellent specificity for detection of Arylsulfatase F (ARSF). No significant cross-reactivity or interference between Arylsulfatase F (ARSF) and analogues was observed.

    Sensitivität

    0.112 ng/mL
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer

    3 h

    Plattentyp

    Pre-coated

    Protokoll

    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase F (ARSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase F (ARSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase F (ARSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylsulfatase F (ARSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arylsulfatase F (ARSF) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arylsulfatase F (ARSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    Haltbarkeit

    4-8 months
  • Target Alle ARSF Produkte

    ARSF (Arylsulfatase F (ARSF))

    Andere Bezeichnung

    Arylsulfatase F

    Hintergrund

    Gene Name: Arylsulfatase F

    Gene Aliases: ASF

    Gen-ID

    416

    UniProt

    P54793
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