Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

beta Arrestin 1 ELISA Kit

ARRB1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5652019
  • Target Alle beta Arrestin 1 (ARRB1) ELISA Kits anzeigen
    beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))
    Reaktivität
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Arrestin Beta 1 (ARRb1). No significant cross-reactivity or interference between Arrestin Beta 1 (ARRb1) and analogues was observed.
    Sensitivität
    0.053 ng/mL
    Featured
    Zu unserem meistverkauften ARRB1 ELISA Kit
    Top Product
    Discover our top product ARRB1 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arrestin Beta 1 (ARRb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arrestin Beta 1 (ARRb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arrestin Beta 1 (ARRb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arrestin Beta 1 (ARRb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arrestin Beta 1 (ARRb1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arrestin Beta 1 (ARRb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle beta Arrestin 1 (ARRB1) ELISA Kits anzeigen
    beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))
    Andere Bezeichnung
    Arrestin Beta 1 (ARRB1 Produkte)
    Synonyme
    arrb1 ELISA Kit, MGC83787 ELISA Kit, arb1 ELISA Kit, arr1 ELISA Kit, barr1 ELISA Kit, betaarr1 ELISA Kit, 1200006I17Rik ELISA Kit, AW208571 ELISA Kit, G430100A01Rik ELISA Kit, ARB1 ELISA Kit, ARR1 ELISA Kit, BARRES ELISA Kit, arrestin beta 1 ELISA Kit, arrestin, beta 1 ELISA Kit, arrestin beta 1 L homeolog ELISA Kit, beta-arrestin 1 ELISA Kit, putative beta-arrestin 1 ELISA Kit, ARRB1 ELISA Kit, arrb1 ELISA Kit, arrb1.L ELISA Kit, CpipJ_CPIJ016988 ELISA Kit, CpipJ_CPIJ017114 ELISA Kit, Smp_122900 ELISA Kit, Smp_126080 ELISA Kit, Smp_176980 ELISA Kit, Smp_152290 ELISA Kit, Arrb1 ELISA Kit
    Hintergrund

    Gene Name: Arrestin Beta 1

    Gene Aliases: ARB1, ARR1, ARR-B1, Arrestin 2

    Pathways
    Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
Sie sind hier:
Kundenservice