RARS ELISA Kit

Produktnummer ABIN5652016

Produktdetails RARS ELISA Kit

Target: RARS (Arginyl-tRNA Synthetase (RARS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Arginyl tRNA Synthetase (RARS). No significant cross-reactivity or interference between Arginyl tRNA Synthetase (RARS) and analogues was observed.

Sensitivität: 0.3 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginyl tRNA Synthetase (RARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginyl tRNA Synthetase (RARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginyl tRNA Synthetase (RARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginyl tRNA Synthetase (RARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginyl tRNA Synthetase (RARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginyl tRNA Synthetase (RARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RARS

Target: RARS (Arginyl-tRNA Synthetase (RARS))

Andere Bezeichnung: Arginyl tRNA Synthetase (RARS Produkte)

Synonyme: ArgRS ELISA Kit, CG9020 ELISA Kit, Dmel\\CG9020 ELISA Kit, RRS ELISA Kit, wu:fa96g09 ELISA Kit, zgc:56050 ELISA Kit, zgc:86730 ELISA Kit, RARS ELISA Kit, xcc-b100_4045 ELISA Kit, F28G11.14 ELISA Kit, F28G11_14 ELISA Kit, ECK1877 ELISA Kit, JW1865 ELISA Kit, lov ELISA Kit, 2610011N19Rik ELISA Kit, 2610037E21Rik ELISA Kit, AL033339 ELISA Kit, AW985894 ELISA Kit, DALRD1 ELISA Kit, ARGRS ELISA Kit, Arginyl-tRNA synthetase ELISA Kit, arginyl-tRNA synthetase ELISA Kit, hypothetical protein ELISA Kit, arginyL-tRNA synthetase ELISA Kit, Arginyl-tRNA synthetase, class Ic ELISA Kit, arginine--tRNA ligase ELISA Kit, ArgRS ELISA Kit, rars ELISA Kit, RARS ELISA Kit, ZMO0843 ELISA Kit, TP01_1024 ELISA Kit, Mmc1_0704 ELISA Kit, Oter_2566 ELISA Kit, argS ELISA Kit, TSTA_030410 ELISA Kit, PAAG_11057 ELISA Kit, MCYG_01632 ELISA Kit, Arch_0351 ELISA Kit, VDIS_RS05935 ELISA Kit, LOC100281742 ELISA Kit, Calkr_0529 ELISA Kit, Palpr_2492 ELISA Kit, Mspyr1_17280 ELISA Kit, AT1G66530 ELISA Kit, UCYN_05320 ELISA Kit, Rars ELISA Kit

Hintergrund:

Gene Name: Arginyl tRNA Synthetase

Gene Aliases: ArgRS, DALRD1, Arginine tRNA Ligase 1,Cytoplasmic

Gen-ID: 5917

UniProt: P54136