Vasopressin ELISA Kit
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- Target Alle Vasopressin (AVP) ELISA Kits anzeigen
- Vasopressin (AVP) (Arginine Vasopressin (AVP))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Competition ELISA
- Detektionsbereich
- 12.35 pg/mL - 1000 pg/mL
- Untere Nachweisgrenze
- 12.35 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Antidiuretic Hormone (ADH). No significant cross-reactivity or interference between Antidiuretic Hormone (ADH) and analogues was observed.
- Sensitivität
- 5.38 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 h
- Plattentyp
- Pre-coated
- Protokoll
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Antidiuretic Hormone (ADH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Antidiuretic Hormone (ADH) and unlabeled Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Antidiuretic Hormone (ADH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Antidiuretic Hormone (ADH) in the sample.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle Vasopressin (AVP) ELISA Kits anzeigen
- Vasopressin (AVP) (Arginine Vasopressin (AVP))
- Andere Bezeichnung
- Antidiuretic Hormone (AVP Produkte)
- Synonyme
- ADH ELISA Kit, ARVP ELISA Kit, AVP-NPII ELISA Kit, AVRP ELISA Kit, VP ELISA Kit, 2610201A18Rik ELISA Kit, Akr1a4 ELISA Kit, Vp ELISA Kit, Vsp ELISA Kit, DI ELISA Kit, Vas ELISA Kit, Adh ELISA Kit, Adh1a ELISA Kit, Adh1c ELISA Kit, adh ELISA Kit, arvp ELISA Kit, avrp ELISA Kit, avp-npii ELISA Kit, copeptin ELISA Kit, vasotocin ELISA Kit, arginine vasopressin ELISA Kit, aldo-keto reductase family 1, member A1 (aldehyde reductase) ELISA Kit, alcohol dehydrogenase 1 (class I) ELISA Kit, AVP ELISA Kit, Akr1a1 ELISA Kit, Avp ELISA Kit, Adh1 ELISA Kit, avp ELISA Kit
- Hintergrund
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Gene Name: Antidiuretic Hormone
Gene Aliases: ARVP, AVP, VP, Arginine Vasopressin, Arg-Vasopressin, Antidiuretic Hormone, Diabetes Insipidus, Neurohypophyseal, Argipressin
- Pathways
- cAMP Metabolic Process
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