AMACR ELISA Kit

Produktnummer ABIN5651657

Produktdetails AMACR ELISA Kit

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Alpha-Methylacyl Coenzyme A Racemase (aMACR). No significant cross-reactivity or interference between Alpha-Methylacyl Coenzyme A Racemase (aMACR) and analogues was observed.

Sensitivität: 0.066 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Methylacyl Coenzyme A Racemase (aMACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Methylacyl Coenzyme A Racemase (aMACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Methylacyl Coenzyme A Racemase (aMACR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Methylacyl Coenzyme A Racemase (aMACR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Andere Bezeichnung: Alpha-Methylacyl Coenzyme A Racemase (AMACR Produkte)

Synonyme: AMACR ELISA Kit, DKFZp469O1232 ELISA Kit, amacr ELISA Kit, NCU04099.1 ELISA Kit, MGC89832 ELISA Kit, AMACRD ELISA Kit, CBAS4 ELISA Kit, RACE ELISA Kit, RM ELISA Kit, Macr1 ELISA Kit, Da1-8 ELISA Kit, Marc1 ELISA Kit, alpha-methylacyl-CoA racemase ELISA Kit, PROBABLE ALPHA-METHYLACYL-COA RACEMASE MCR (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) ELISA Kit, Alpha-methylacyl-CoA racemase ELISA Kit, alpha-methylacyl-CoA racemase L homeolog ELISA Kit, AMACR ELISA Kit, mcr ELISA Kit, BPSL0064 ELISA Kit, Maqu_2037 ELISA Kit, UREG_03852 ELISA Kit, MCYG_01809 ELISA Kit, amacr ELISA Kit, amacr.L ELISA Kit, MGYG_04259 ELISA Kit, NCU04099 ELISA Kit, Amacr ELISA Kit

Hintergrund:

Gene Name: Alpha-Methylacyl Coenzyme A Racemase

Gene Aliases: RACE, 2-methylacyl-CoA racemase

Gen-ID: 23600

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process