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ADRB3 ELISA Kit

ADRB3 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5651479
  • Target Alle ADRB3 ELISA Kits anzeigen
    ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))
    Reaktivität
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Beta 3 (ADRb3). No significant cross-reactivity or interference between Adrenergic Receptor Beta 3 (ADRb3) and analogues was observed.
    Sensitivität
    0.062 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta 3 (ADRb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Beta 3 (ADRb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta 3 (ADRb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Beta 3 (ADRb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Beta 3 (ADRb3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Beta 3 (ADRb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ADRB3 ELISA Kits anzeigen
    ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))
    Andere Bezeichnung
    Adrenergic Receptor Beta 3 (ADRB3 Produkte)
    Synonyme
    BETA3AR ELISA Kit, Adrb-3 ELISA Kit, ADRB ELISA Kit, BAR3 ELISA Kit, B3AR ELISA Kit, GPCR ELISA Kit, im:7154935 ELISA Kit, si:ch211-215f19.1 ELISA Kit, zgc:194843 ELISA Kit, zgc:194852 ELISA Kit, adrenoceptor beta 3 ELISA Kit, adrenergic receptor, beta 3 ELISA Kit, adrenoceptor beta 3a ELISA Kit, adrenoceptor beta 3b ELISA Kit, ADRB3 ELISA Kit, Adrb3 ELISA Kit, adrb3a ELISA Kit, adrb3b ELISA Kit
    Hintergrund

    Gene Name: Adrenergic Receptor Beta 3

    Gene Aliases: BETA3AR, B3AR, Beta-3 adrenoreceptor

    Pathways
    cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation
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