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Adiponectin Receptor 1 ELISA Kit

ADIPOR1 Reaktivität: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5651455
  • Target Alle Adiponectin Receptor 1 (ADIPOR1) ELISA Kits anzeigen
    Adiponectin Receptor 1 (ADIPOR1)
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.781 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.781 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Adiponectin Receptor 1 (ADIPOR1). No significant cross-reactivity or interference between Adiponectin Receptor 1 (ADIPOR1) and analogues was observed.
    Sensitivität
    0.29 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin Receptor 1 (ADIPOR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin Receptor 1 (ADIPOR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin Receptor 1 (ADIPOR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin Receptor 1 (ADIPOR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin Receptor 1 (ADIPOR1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin Receptor 1 (ADIPOR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Adiponectin Receptor 1 (ADIPOR1) ELISA Kits anzeigen
    Adiponectin Receptor 1 (ADIPOR1)
    Andere Bezeichnung
    Adiponectin Receptor 1 (ADIPOR1 Produkte)
    Synonyme
    ADIPOR1 ELISA Kit, adipor1 ELISA Kit, zgc:92893 ELISA Kit, ACDCR1 ELISA Kit, CGI45 ELISA Kit, PAQR1 ELISA Kit, TESBP1A ELISA Kit, 2810031L11Rik ELISA Kit, CGI-45 ELISA Kit, Paqr1 ELISA Kit, zgc:55386 ELISA Kit, wu:fj02d08 ELISA Kit, acdcr1 ELISA Kit, cgi-45 ELISA Kit, cgi45 ELISA Kit, paqr1 ELISA Kit, tesbp1a ELISA Kit, MGC53458 ELISA Kit, Adipor1 ELISA Kit, adiponectin receptor 1 ELISA Kit, adiponectin receptor 1a ELISA Kit, adiponectin receptor 1b ELISA Kit, adiponectin receptor 1 S homeolog ELISA Kit, adiponectin receptor 1 L homeolog ELISA Kit, ADIPOR1 ELISA Kit, adipor1a ELISA Kit, Adipor1 ELISA Kit, adipor1b ELISA Kit, adipor1 ELISA Kit, adipor1.S ELISA Kit, adipor1.L ELISA Kit
    Hintergrund

    Gene Name: Adiponectin Receptor 1

    Gene Aliases: ACDCR1, CGI45, PAQR1, TESBP1A, TESBP1-A, Progestin and AdipoQ Receptor Family Member I

    Gen-ID
    51094
    UniProt
    Q96A54
    Pathways
    AMPK Signaling
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