PACAP ELISA Kit

Produktnummer ABIN5651436

Produktdetails PACAP ELISA Kit

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.81 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.

Sensitivität: 3.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PACAP

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Andere Bezeichnung: Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1 Produkte)

Synonyme: PACAP ELISA Kit, grf ELISA Kit, Pacap ELISA Kit, adcyap1 ELISA Kit, GRF-PACAP ELISA Kit, zgc:103760 ELISA Kit, xx:af217251 ELISA Kit, pacap ELISA Kit, ADCYAP1 ELISA Kit, PACAP2 ELISA Kit, acap1l ELISA Kit, pacap1b ELISA Kit, wu:fj30e08 ELISA Kit, zgc:101522 ELISA Kit, zgc:92555 ELISA Kit, adenylate cyclase activating polypeptide 1 ELISA Kit, adenylate cyclase activating polypeptide 1a ELISA Kit, adenylate cyclase activating polypeptide 1 (pituitary) ELISA Kit, adenylate cyclase activating polypeptide 1b ELISA Kit, ADCYAP1 ELISA Kit, Adcyap1 ELISA Kit, adcyap1a ELISA Kit, adcyap1 ELISA Kit, adcyap1b ELISA Kit

Hintergrund:

Gene Name: Adenylate Cyclase Activating Polypeptide 1, Pituitary

Gene Aliases: PACAP, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

Gen-ID: 11516

UniProt: O70176

Pathways: Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling