PACAP ELISA Kit

Produktnummer ABIN5651435

Human PACAP ELISA Kit, Colorimetric Assay für die Quantifizierung von Human PACAP.

Kurzübersicht für PACAP ELISA Kit (ABIN5651435)

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 6.17 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails PACAP ELISA Kit

Untere Nachweisgrenze: 6.17 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.

Sensitivität: 2.55 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and unlabeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) (Standards or samples) with the pre-coated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PACAP

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Andere Bezeichnung: Adenylate Cyclase Activating Polypeptide 1, Pituitary

Hintergrund:

Gene Name: Adenylate Cyclase Activating Polypeptide 1, Pituitary

Gene Aliases: PACAP, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

Gen-ID: 116

UniProt: P18509

Pathways: Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling